|
同位素示踪法测定125I -NGF在小鼠体内的血药浓度变化
|
|
|
| 摘 要：本文涉及应用Idogn法对神经生长因子（NGF）进行125I标记并利用同位素示踪与电泳分离相结合的方法测定125I -NGF在小鼠体内的血药浓度与时间变化关系。结果显示，静脉注射125I-NGF在小鼠体内的代谢规律符合二房室开放模型。分布相半衰期为0.13h，消除相半衰期为3.68h，125I -NGF在小鼠体内分布和消除均较快，清除率为0.125L/h/Kg，表观分布容积为0.697L/kg，曲线所围面积为16.01μg·h/L。 |
| 关键词：神经生长因子,125I标记,同位素示踪法,十二烷基硫酸钠-聚丙烯酰胺凝胶电泳 |
|
文章全文为PDF格式，请下载到本机浏览。[下载全文]
如您没有PDF阅读器，请先下载PDF阅读器 Acrobat Reader [下载阅读器]
|
|
| Determination of the change of 125I -NGF concentration in plasma in mouse |
|
|
| Abstract：The study concerns in the label of nerve growth factor(NGF)by 125I with Idogn method.The change of 125I -NGF Concentration in Plasma in Mouse with time was determinated using the method of sodium dodecylsilfate-poly-accrylamide gel electrophoresis(SDS PAGE)after iv 125I -NGF.Result demonstrate the time 125I -NGF concentration in plasma curve after iv fit a two compartment open model.Distribution phase half life is 0.13h，elimination phase half life is 3.68h，the mean plasma clearance is 0.125L/（h·Kg），apparent volume of distribution is 0.697L/kg，area under curve is 16.012μg·h/L. |
| Key words：Nerve growth factor(NGF),Isotope tracing method,SDS |
| 【大 中 小】
[关闭窗口] |
| |
